LOCUS       V005188                 9266 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005188
VERSION     V005188
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Cloning vector pKS300IC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2016) Contact:Asao Fujiyama National Instetute of
            Genetics, Center for Information Biology; 1111 Yata, Mishima,
            Shizuoka 411-8540, Japan URL :http://www.nig.ac.jp/
REFERENCE   3  (bases 1 to 9266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2016) Contact:Asao Fujiyama National Instetute of Genetics,
            Center for Information Biology; 1111 Yata, Mishima, Shizuoka
            411-8540, Japan URL :http://www.nig.ac.jp/"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..108)
                     /label="pKS300IC_Rev"
                     /note="pKS300IC_Rev"
     promoter        160..264
                     /label="AmpR promoter"
     CDS             265..1122
                     /label="AmpR"
                     /note="beta-lactamase"
     misc_feature    complement(1610..2083)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2159..3127)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(3130..4302)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(4628..4878)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4884..5636)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(5727..5946)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             complement(6413..7216)
                     /gene="resD"
                     /label="Resolvase"
                     /note="Resolvase from Escherichia coli (strain K12).
                     Accession#: P06615"
     CDS             complement(7220..7522)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(7527..7742)
                     /gene="ccdA"
                     /label="Antitoxin CcdA"
                     /note="Antitoxin CcdA from Escherichia coli (strain K12).
                     Accession#: P62552"
     primer_bind     8097..8117
                     /label="pKS300IC_Fwd"
                     /note="pKS300IC_Fwd"
ORIGIN
        1 gttaacgttt aaacgattcg aatccgtaac tataacggtc ctaaggtagc gaagaattct
       61 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      121 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      181 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      241 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      301 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      361 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      421 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      481 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
      541 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      601 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      661 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      721 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      781 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
      841 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      901 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      961 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1021 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1081 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1141 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1201 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctacgcgta
     1321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1381 gagctaccag cggtggtttg tttaacgtgc cggcacggcc tgggtaacca ggtattttgt
     1441 ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat ccacagcagg
     1501 catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg tcaatacttg
     1561 cccttgacag gcattgatgg aatcgtagtc tcacgctgat agtctgatcg acaatacaag
     1621 tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggatc gtggtcccag
     1681 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
     1741 cgatacgagt gggaccgtgg ttccagacta ataatcagac cgacgatacg agtgggaccg
     1801 tggtcccaga ctaataatca gaccgacgat acgagtggga ccatggtccc agactaataa
     1861 tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc gacgatacga
     1921 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca
     1981 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
     2041 acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg ctttctggcc
     2101 tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg tcgcatcagg
     2161 gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac tcagttggaa
     2221 cacgagacct gtccaggtta agcaccattt tatcgccctt atacaatact gtcgctccag
     2281 gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt ttaagcacag
     2341 aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt ttctgctcat
     2401 gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct ttttgaagtg
     2461 catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca actgatttag
     2521 gcaatttggc ggtgttgata cagcgggtaa taatcttacg tgaaatattt tccgcatcag
     2581 ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa cgctgaccac
     2641 gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca gccatctgct
     2701 catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca gcagctttac
     2761 gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg aacgccggtg
     2821 tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc tcagtaagca
     2881 gctcctggtc acgttcatta cctgaccata cccgagaggt cttctcaaca ctatcacccc
     2941 ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta atggcattac
     3001 cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca gctggtgtcg
     3061 ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata acaggcgcac
     3121 gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt cattgcagac
     3181 aggttcccaa atagaaagag catttctcca ggcaccagtt gaagagcgtt gatcaatggc
     3241 ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg tttcacgtac
     3301 aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca tccacgggga
     3361 ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag gctcgaaccc
     3421 tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca gtgcggaggt
     3481 gtagtcaaac aactcagcag gcgtgggaac aatcagcaca tcagcagcac atacgacatt
     3541 aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat agtcatgagc
     3601 aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt taccttcatc
     3661 aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag gaataatgtc
     3721 aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt ccccaagata
     3781 gaaaggcagg agagtgtctt ctgcatgaat atgaagatct ggtacccatc cgtgatacat
     3841 tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc ccttcagagc
     3901 cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt tatgggcagc
     3961 aaccccgatc accggtggaa atacgtcttc agcacgtcgc aatcgcgtac caaacacatc
     4021 acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc gtcctcgaat
     4081 ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag cctgagaaga
     4141 aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt tcctcgcttc
     4201 cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat gaccagcgtt
     4261 tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat cattgctttg
     4321 cgttttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc aaagtcatca
     4381 aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa gaagagcaca
     4441 tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag catagcgagc
     4501 gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc tcagcgcaag
     4561 aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat agccaattca
     4621 gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc ccgatatcag
     4681 gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct caaatttggc
     4741 ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga aggaaacagc
     4801 aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca ccctcaaatc
     4861 tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgatatcgcg gaaggaaaat
     4921 acgatatgag tcgtctggcg gcctttcttt ttctcaatgt atgagaggcg cattggagtt
     4981 ctgctgttga tctcattaac acagacctgc aggaagcggc ggcggaagtc aggcatacgc
     5041 tggtaacttt gaggcagctg gtaacgctct atgatccagt cgattttcag agagacgatg
     5101 cctgagccat ccggcttacg atactgacac agggattcgt ataaacgcat ggcatacgga
     5161 ttggtgattt cttttgtttc actaagccga aactgcgtaa accggttctg taacccgata
     5221 aagaagggaa tgagatatgg gttgatatgt acactgtaaa gccctctgga tggactgtgc
     5281 gcacgtttga taaaccaagg aaaagattca tagccttttt catcgccggc atcctcttca
     5341 gggcgataaa aaaccacttc cttccccgcg aaactcttca atgcctgccg tatatcctta
     5401 ctggcttccg cagaggtcaa tccgaatatt tcagcatatt tagcaacatg gatctcgcag
     5461 ataccgtcat gttcctgtag ggtgccatca gattttctga tctggtcaac gaacagatac
     5521 agcatacgtt tttgatcccg ggagagacta tatgccgcct cagtgaggtc gtttgactgg
     5581 acgattcgcg ggctattttt acgtttcttg tgattgataa ccgctgtttc cgccatgaca
     5641 gatccatgtg aagtgtgaca agtttttaga ttgtcacact aaataaaaaa gagtcaataa
     5701 gcagggataa ctttgtgaaa aaacagcttc ttctgagggc aatttgtcac agggttaagg
     5761 gcaatttgtc acagacagga ctgtcatttg agggtgattt gtcacactga aagggcaatt
     5821 tgtcacaaca ccttctctag aaccagcatg gataaaggcc tacaaggcgc tctaaaaaag
     5881 aagatctaaa aactataaaa aaaataatta taaaaatatc cccgtggata agtggataac
     5941 cccaagggaa gttttttcag gcatcgtgtg taagcagaat atataagtgc tgttccctgg
     6001 tgcttcctcg ctcactcgag ggcttcgccc tgtcgctcga ctgcggcgag cactactggc
     6061 tgtaaaagga cagaccacat catggttctg tgttcattag gttgttctgt ccattgctga
     6121 cataatccgc tccacttcaa cgtaacaccg cacgaagatt tctattgttc ctgaaggcat
     6181 attcaaatcg ttttcgttac cgcttgcagg catcatgaca gaacactact tcctataaac
     6241 gctacacagg ctcctgagat taataatgcg gatctctacg ataatgggag attttcccga
     6301 ctgtttcgtt cgcttctcag tggataacag ccagcttctc tgtttaacag acaaaaacag
     6361 catatccact cagttccaca tttccatata aaggccaagg catttattct caggataatt
     6421 gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg tgccgggcag
     6481 ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga actgatggac ttatgtccca
     6541 tcaggctttg cagaactttc agcggtatac cggcatacag catgtgcatc gcataggaat
     6601 ggcggaacgt atgtggtgtg accggaacag agaacgtcac accgtcagca gcagcggcgg
     6661 caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt
     6721 tctctgtcct tcctgtgcga cggttacgcc gctccatggg tattttcagt gttgccacca
     6781 tcgtctgcag ctggctgacg taccaggagt cagagagcgg aaccagccgg tgagtctgct
     6841 gaccggcggg cattctcccc gccgtcctgg cagctttttc ggtccgttgt ttcagggtcg
     6901 caagctgcac aaacggatac ggaggcgcaa gcgaaaaatc cccccgcgtc agcgccagtg
     6961 cttcattaat gcgtgctccg gtgttccaca gtgtggccag cagcatcttg cggtgcagat
     7021 ccgggacgta atggagcagg gcactcactt ccggagccag cagatatttt ggcagttcat
     7081 catggaccat cgacatctgg cgaagtgcca gagctgccgg ataatcaatg gcaacaggca
     7141 gcgatgcagg ctgcccggca gaatacactg ccggtaccat gactgcagac tggctgtgta
     7201 taacggagcc tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
     7261 tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca
     7321 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
     7381 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
     7441 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
     7501 aggtgtaaac cttaaactgc atttcaccag tccctgttct cgtcagcaaa agagccgttc
     7561 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
     7621 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
     7681 tcatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
     7741 atagcacacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
     7801 aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagcct tatgtatttt
     7861 acctttcgtt atgttccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     7921 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     7981 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     8041 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     8101 actgggttga aggctctcaa gggcatcggt cgaggaactt tcggcggctt tgctgtgcga
     8161 caggctcacg tctaaaagga aataaatcat gggtcataaa attatcacgt tgtccggcgc
     8221 ggcgacggat gttctgtatg cgctgttttt ccgtggcgcg ttgctgtctg gtgatctgcc
     8281 ttctaaatct ggcacagccg aattgcgcga gcttggtttt gctgaaacca gacacacagc
     8341 aactgaatac cagaaagaaa atcactttac ctttctgaca tcagaagggc agaaatttgc
     8401 cgttgaacac ctggtcaata cgcgttttgg tgagcagcaa tattgcgctt cgatgacgct
     8461 tggcgttgag attgatacct ctgctgcaca aaaggcaatc gacgagctgg accagcgcat
     8521 tcgtgacacc gtctccttcg aacttattcg caatggagtg tcattcatca aggacgccgc
     8581 tatcgcaaat ggtgctatcc acgcagcggc aatcgaaaca cctcagccgg tgaccaatat
     8641 ctacaacatc agccttggta tccagcgtga tgagccagcg cagaacaagg taaccgtcag
     8701 tgccgataag ttcaaagtta aacctggtgt tgataccaac attgaaacgt tgatcgaaaa
     8761 cgcgctgaaa aacgctgctg aatgtgcggc gctggatgtc acaaagcaaa tggcagcaga
     8821 caagaaagcg atggatgaac tggcttccta tgtccgcacg gccatcatga tggaatgttt
     8881 ccccggtggt gtcatctggc agcagtgccg tcgatagtat gcaattgata attattatca
     8941 tttgcgggtc ctttccggcg atccgccttg ttacggggcg gcgacctcgc gggttttcgc
     9001 tatttatgaa aattttccgg tttaaggcgt ttccgttctt cttcgtcata acttaatgtt
     9061 tttatttaaa ataccctctg aaaagaaagg aaacgacagg tgctgaaagc gagctttttg
     9121 gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcaacaaaa
     9181 agcagagctt atcgatgata agcggtcaaa catgagaatt cttcgctacc ttaggaccgt
     9241 tatagttacg gatgatcatc cacgtg
//
